منابع مشابه
Evidence for distinct mechanisms facilitating transcript elongation through chromatin in vivo.
The mechanism and kinetics of RNA polymerase II transcription and histone acetylation were studied by chromatin immunoprecipitation in yeast. Our results indicate that a significant fraction of polymerases starting transcription never make it to the end of a long GAL-VPS13 fusion gene. Surprisingly, induction of GAL genes results in substantial loss of histone-DNA contacts not only in the promo...
متن کاملMechanisms of elongation in embryogenesis.
Here, I discuss selected examples of elongation in embryogenesis to identify common and unique mechanisms, useful questions for further work, and new systems that offer opportunities for answering these questions. Fiber-wound, hydraulic mechanisms of elongation highlight the importance of biomechanical linkages of otherwise unrelated cellular behaviors during elongation. Little-studied examples...
متن کاملFACT, a Factor that Facilitates Transcript Elongation through Nucleosomes
The requirements for transcriptional activation by RNA polymerase II were examined using chromatin templates assembled in vitro and a transcription system composed of the human general transcription factors and RNA polymerase II. Activator-induced, energy-dependent chromatin remodeling promoted efficient preinitiation complex formation and transcription initiation, but was not sufficient for pr...
متن کاملA Real Stretch: Mechanisms Behind Cell Elongation
Does tightening your belt make you taller? You might be tempted to conclude so after learning the results of an intriguing study of how notochord cells elongate in embryos of a primitive sea creature. Embryonic development involves two basic processes: cell multiplication and cell shape changes. Elongation in particular is a crucial process for development of the notochord, which serves as a ‘‘...
متن کاملTranscription elongation: structural basis and mechanisms.
A ternary complex composed of RNA polymerase (RNAP), DNA template, and RNA transcript is the central intermediate in the transcription cycle responsible for the elongation of the RNA chain. Although the basic biochemistry of RNAP functioning is well understood, little is known about the underlying structural determinants. The absence of high- resolution structural data has hampered our understa...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.430.1